Western Blot using anti-CCR4 antibody KW-0761 (Mogamulizumab) (Ab00726) Human brain cerebellum (A) and human heart (B) lysates (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of KW-0761 (Ab00726-23.0) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CCR4 is 37.7kDa.